Prolyl Endopeptidase Antibody / PREP

Prolyl endopeptidase (PE) also known as prolyl oligopeptidase or post-proline cleaving enzyme is an enzyme that in humans is encoded by the PREP gene. The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P48147

Host

Rabbit

Reactivity

Human, Rat

Immunogen

An E. coli-derived human protein (amino acids M1-E227) was used as the immunogen for the Prolyl Endopeptidase antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Prolyl Endopeptidase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Prolyl Endopeptidase antibody is available for research use only.

Storage Conditions

After reconstitution, the Prolyl Endopeptidase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Prolyl Endopeptidase antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human skin cancer tissue with Prolyl Endopeptidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.