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PPP4R1 Antibody / PP4R1

Serine/threonine-protein phosphatase 4 regulatory subunit 1 is an enzyme that in humans is encoded by the PPP4R1 gene. This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4) . The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470) . There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q8TF05

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Amino acids HQEAVEQTIMALQMDRDSDVKYFASIH from the human protein were used as the immunogen for the PPP4R1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the PPP4R1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PPP4R1 antibody is available for research use only.

Storage Conditions

After reconstitution, the PPP4R1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PPP4R1 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human A431 cells with PPP4R1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= PPP4R1 antibody.
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