Astrin Antibody / SPAG5

Sperm-associated antigen 5, also called Astrin and Deepest, is a protein that in humans is encoded by the SPAG5 gene. This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q96R06

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids E738-S1193) was used at the immunogen for the Astrin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Astrin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Astrin antibody is available for research use only.

Storage Conditions

After reconstitution, the Astrin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Astrin antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human rectal cancer tissue with Astrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.