Glutamate-oxaloacetate transaminase 2 Antibody / GOT2 / FABP-1

Aspartate aminotransferase, mitochondrial is an enzyme that in humans is encoded by the GOT2 gene. Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P00505

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids H35-K430) was used as the immunogen for the Glutamate-oxaloacetate transaminase 2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Glutamate-oxaloacetate transaminase 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Glutamate-oxaloacetate transaminase 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Glutamate-oxaloacetate transaminase 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Glutamate-oxaloacetate transaminase 2 antibody should be determined by the researcher.

Location

Cytoplasmic, cell membrane

Image Legend

IHC staining of FFPE human ovarian cancer tissue with Glutamate-oxaloacetate transaminase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.