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HFH-4 Antibody / FOXJ1

Forkhead box protein J1, also called Hepatocyte nuclear factor 3 forkhead homolog 4 or HFH-4, is a protein that in humans is encoded by the FOXJ1 gene. This gene encodes a member of the forkhead family of transcription factors. Similar genes in zebrafish and mouse have been shown to regulate the transcription of genes that control the production of motile cilia. The mouse ortholog also functions in the determination of left-right asymmetry. Polymorphisms in this gene are associated with systemic lupus erythematosus and allergic rhinitis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q92949

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Recombinant human protein (amino acids D27-A405) was used as the immunogen for the HFH-4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the HFH-4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HFH-4 antibody is available for research use only.

Storage Conditions

After reconstitution, the HFH-4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HFH-4 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE rat ovary tissue with HFH-4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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