Clathrin heavy chain Antibody / CLTC

Clathrin heavy chain 1 is a protein that in humans is encoded by the CLTC gene. Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q00610

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein (amino acids R967-Q1668) was used as the immunogen for the Clathrin heavy chain antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

6D3

Applications

WB, IHC-P, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Clathrin heavy chain antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Clathrin heavy chain antibody is available for research use only.

Storage Conditions

After reconstitution, the Clathrin heavy chain antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Clathrin heavy chain antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Flow cytometry testing of human HepG2 cells with Clathrin heavy chain antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= Clathrin heavy chain antibody.