SHMT Antibody / SHMT1
This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P34896
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Recombinant human protein (amino acids M1-S470) was used as the immunogen for the SHMT antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
9C7
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity purified
Format
Purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
Limitations
This SHMT antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the SHMT antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human liver cancer tissue with SHMT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Frequently Asked Questions
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