Indoleamine 2,3-dioxygenase 2 Antibody / IDO2 / INDOL1

IDO2 (Indoleamine 2,3-dioxygenase 2), also called INDOLEAMINE 2,3-DIOXYGENASE-LIKE 1 or INDOL1, is an enzyme encoded by the INDOL1 gene which metabolizes tryptophan in the kynurenine pathway. By genomic sequence analysis, the INDOL1 gene is mapped on chromosome 8p12 just downstream of the INDO gene. And its exact cytogenetic location is 8p11.21. By database analysis using INDO as probe, followed by RT-PCR of total RNA from various tissues, IDO2 is cloned by human and mouse INDOL1. INDOL1 catabolizes tryptophan as determined by Kyn production, but unlike INDO, is inhibited by D-1-methyl-tryptophan (D-1MT) but not the L-1MT stereoisomer. The Gene Structure of the INDOL1 has 11 exons and spans 74 kb.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q6ZQW0

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant human protein (amino acids M1-R357) was used as the immunogen for the Indoleamine 2,3-dioxygenase 2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Indoleamine 2,3-dioxygenase 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Indoleamine 2,3-dioxygenase 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Indoleamine 2,3-dioxygenase 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Indoleamine 2,3-dioxygenase 2 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human liver cancer with Indoleamine 2,3-dioxygenase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.