U2AF65 Antibody / U2AF2

Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene. It is mapped to 19q13.42. U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P26368

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant partial protein (amino acids M238-H470) was used as the immunogen for the U2AF65 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

10F4

Applications

WB, IHC-P, IF, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the U2AF65 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This U2AF65 antibody is available for research use only.

Storage Conditions

After reconstitution, the U2AF65 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the U2AF65 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Western blot testing of human 1) HEK293, 2) ThP-1, 3) U-2 OS, 4) Jurkat, 5) rat PC-12, 6) mouse brain, 7) rat brain and 8) mouse RAW264.7 cell lysate with U2AF65 antibody. Expected molecular weight ~65 kDa.