TKT Antibody / Transketolase

Transketolase is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. The pentose phosphate pathway, which is active in most tissues, provides sugar phosphates for intermediary biosynthesis, especially nucleotide metabolism, and generates the biosynthetic reducing power for the cell in the form of NADPH. Transketolase is directly involved in the branch of the pathway that channels excess sugar phosphates to glycolysis, enabling the production of NADPH to be maintained under different metabolic conditions. NADPH is critical for maintaining cerebral glutathione, and thus it is likely that transketolase plays an important role in brain metabolism.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P29401

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant partial protein (amino acids M1-A116) was used as the immunogen for the Transketolase antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

3E5.

Applications

WB, IHC-P, IF, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Transketolase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Transketolase antibody is available for research use only.

Storage Conditions

After reconstitution, the Transketolase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.