PORC-PI-1 Antibody / CDC45

CDC45 is a protein that in humans is encoded by the CDC45 gene. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

O75419

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant partial protein (amino acids E166-A431) was used as the immunogen for the PORC-PI-1 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

6H6

Applications

WB, IHC-P, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the PORC-PI-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This PORC-PI-1 antibody is available for research use only.

Storage Conditions

After reconstitution, the PORC-PI-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the PORC-PI-1 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

IHC staining of FFPE human ovarian cancer with PORC-PI-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.