Poly (ADP-ribose) polymerase 1 Antibody / PARP1

Poly [ADP-ribose] polymerase 1 (PARP1), also known as ADPRT or PPOL is an enzyme that in humans is encoded by the PARP1 gene. PARP1 gene is mapped to 1q42.12. This gene encodes a chromatin-associated enzyme, poly (ADP-ribosyl) transferase, which modifies various nuclear proteins by poly (ADP-ribosyl) ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P09874

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant partial protein (amino acids Q670-R858) was used as the immunogen for the Poly (ADP-ribose) polymerase 1 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

1E12.

Applications

WB, IHC-P, IF, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Poly (ADP-ribose) polymerase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Poly (ADP-ribose) polymerase 1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Poly (ADP-ribose) polymerase 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Poly (ADP-ribose) polymerase 1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human gallbladder adenocarcinoma with Poly (ADP-ribose) polymerase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.