Neurofilament Light Antibody / NF-L

Neurofilament light polypeptide (NFL), also known as neurofilament light chain, is a neurofilament protein that in humans is encoded by the NEFL gene. Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

P07196

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant partial protein (amino acids F4-A463) was used as the immunogen for the Neurofilament Light antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IHC-P, IF, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Neurofilament Light antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Neurofilament Light antibody is available for research use only.

Storage Conditions

After reconstitution, the Neurofilament Light antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Neurofilament Light antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE mouse spinal cord with Neurofilament Light antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.