Neurofilament heavy Antibody / Nefh

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P19246

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

A mouse recombinant partial protein (amino acids Y109-E466) was used as the immunogen for the Neurofilament heavy antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Neurofilament heavy antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Neurofilament heavy antibody is available for research use only.

Storage Conditions

After reconstitution, the Neurofilament heavy antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Neurofilament heavy antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE mouse spinal cord with Neurofilament heavy antibody (right) and negative control (left) . HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.