CSPG4 Antibody / NG2

Chondroitin sulfate proteoglycan 4, also known as melanoma-associated chondroitin sulfate proteoglycan (MCSP) or neuron-glial antigen 2 (NG2), is a chondroitin sulfate proteoglycan that in humans is encoded by the CSPG4 gene. CSPG4 plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q6UVK1

Host

Rabbit

Reactivity

Human

Immunogen

A human recombinant partial protein (amino acids N962-D1194) was used as the immunogen for the CSPG4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the CSPG4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CSPG4 antibody is available for research use only.

Storage Conditions

After reconstitution, the CSPG4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CSPG4 antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) A375 and 2) MCF7 cell lysate with CSPG4 antibody. Predicted molecular weight ~251 kDa but may be seen at higher molecular weights due to glycosylation.