CD133 Antibody / PROM1

Prominin-1, also known as CD133, is a glycoprotein that in humans is encoded by the PROM1 gene. It is mapped to 4p15.32. Prominin-1 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. It has been proposed to act as an organizer of cell membrane topology. Prominin-1 was expressed not only on metastatic colon cancer cells, but also on differentiated colonic epithelium in both adult mice and humans.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL

UniProt

O43490

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant partial protein (amino acids P531-H865) was used as the immunogen for the CD133 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

7F13

Applications

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the CD133 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CD133 antibody is available for research use only.

Storage Conditions

After reconstitution, the CD133 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CD133 antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) Caco-2 and 2) SW620 lysate with PROM1 antibody. Expected molecular weight: 97 kDa-130 kDa depending on glycosylation level.