ERF1 Antibody / EFT1
Eukaryotic translation termination factor 1 (eRF1), also known as TB3-1, is a protein that in humans is encoded by the ETF1 gene. It is mapped to 5q31.2. This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD) . Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P62495
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant partial protein (amino acids D9-K342) was used as the immunogen for the eRF1 antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
3B6
Applications
WB, IHC-P, IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
Limitations
This eRF1 antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the eRF1 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human breast cancer with eRF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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