ERF1 Antibody / EFT1

Eukaryotic translation termination factor 1 (eRF1), also known as TB3-1, is a protein that in humans is encoded by the ETF1 gene. It is mapped to 5q31.2. This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD) . Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P62495

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant partial protein (amino acids D9-K342) was used as the immunogen for the eRF1 antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

3B6

Applications

WB, IHC-P, IF, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the eRF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This eRF1 antibody is available for research use only.

Storage Conditions

After reconstitution, the eRF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the eRF1 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human breast cancer with eRF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

MBS6301016-01 0.2 mL

GAGE2B, NT (GAGE2B, GAGE2, G antigen 2B/2C, Cancer/testis antigen 4.2, G antigen 2C) (AP)

Ask

View Details

MBS6301016-02 5x 0.2 mL

GAGE2B, NT (GAGE2B, GAGE2, G antigen 2B/2C, Cancer/testis antigen 4.2, G antigen 2C) (AP)

Ask

View Details

MBS1201372-01 0.02 mg (E-Coli)

Recombinant Escherichia coli O157:H7 Uncharacterized HTH-type transcriptional regulator ydfH (ydfH)

Ask

View Details

MBS1201372-02 0.1 mg (E-Coli)

Recombinant Escherichia coli O157:H7 Uncharacterized HTH-type transcriptional regulator ydfH (ydfH)

Ask

View Details

MBS1201372-03 0.02 mg (Yeast)

Recombinant Escherichia coli O157:H7 Uncharacterized HTH-type transcriptional regulator ydfH (ydfH)

Ask

View Details

MBS1201372-04 0.1 mg (Yeast)

Recombinant Escherichia coli O157:H7 Uncharacterized HTH-type transcriptional regulator ydfH (ydfH)

Ask

View Details