ACTA1 Antibody
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL
UniProt
P68133
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids HETTYNSIMKCDIDIRKDLYANNVMSGGTTMY from the human protein were used as the immunogen for the ACTA1 antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
3H5
Applications
WB, IHC-P
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
Limitations
This ACTA1 antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ACTA1 antibody should be determined by the researcher.
Image Legend
IHC staining of FFPE human bladder cancer with ACTA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Documents
Frequently Asked Questions
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