UBE2D1/2/3/4 Antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P51668, P62837, P61077, Q9Y2X8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant partial protein (amino acids D116-M147) was used as the immunogen for the UBE2D1/2/3/4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.0125% sodium azide

Reconstitution

After reconstitution, the UBE2D1/2/3/4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This UBE2D1/2/3/4 antibody is available for research use only.

Storage Conditions

After reconstitution, the UBE2D1/2/3/4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the UBE2D1/2/3/4 antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human A431 cells with UBE2D1/2/3/4 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.