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Tspan8 Antibody

Tetraspanin-8 is a protein that in humans is encoded by the TSPAN8 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q8R3G9

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

A mouse recombinant partial protein (amino acids A108-N203) was used as the immunogen for the Tspan8 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IHC-P, IF, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.0125% sodium azide

Reconstitution

After reconstitution, the Tspan8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Tspan8 antibody is available for research use only.

Storage Conditions

After reconstitution, the Tspan8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Tspan8 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE mouse stomach with Tspan8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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