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SUMO1 Antibody

Small ubiquitin-related modifier 1 (SUMO1), also called SMT3C or PIC1 is a protein that in humans is encoded by the SUMO1 gene. This gene is mapped to 2q33.1. This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P63165

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids HLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKEL from the human protein were used as the immunogen for the SUMO1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, IF, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.0125% sodium azide

Reconstitution

After reconstitution, the SUMO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SUMO1 antibody is available for research use only.

Storage Conditions

After reconstitution, the SUMO1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SUMO1 antibody should be determined by the researcher.

Location

Predominantly nuclear with some cytoplasmic

Image Legend

IHC staining of FFPE human breast cancer with SUMO1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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