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METTL1 Antibody

TRNA (guanine-N (7) -) -methyltransferase is an enzyme that in humans is encoded by the METTL1 gene. This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9UBP6

Host

Rabbit

Reactivity

Human, Rat

Immunogen

A human recombinant partial protein (amino acids A2-H276) was used as the immunogen for the METTL1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the METTL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This METTL1 antibody is available for research use only.

Storage Conditions

After reconstitution, the METTL1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the METTL1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human breast cancer with METTL1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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