Fadd Antibody

FADD, Fas-Associated protein with Death Domain, is a universal adaptor protein in apoptosis that mediates signaling of all known death domain-containing members of the TNF receptor superfamily. The FADD gene contains 2 exons and spans approximately 3.6 kb. By analysis of somatic cell hybrid panels and by fluorescence in situ hybridization, the FADD gene is mapped to 11q13.3. The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, thus, it participates in the death signaling initiated by these receptors.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q61160

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Amino acids AEKKNASVAGLVKALRTCRLNLVADLVEEAQES from the mouse protein were used as the immunogen for the Fadd antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.0125% sodium azide

Reconstitution

After reconstitution, the Fadd antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Fadd antibody is available for research use only.

Storage Conditions

After reconstitution, the Fadd antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Fadd antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of rat 1) brain, 2) stomach, 3) lung, 4) liver, 5) kidney, 6) spleen, 7) thymus and 8) PC-12 lysate with Fadd antibody. Predicted molecular weight ~23 kDa, commonly observed at 23-28 kDa.