CDK12 Antibody / Cyclin-dependent kinase 12

CDK12 (Cyclin-dependent kinase 12) is a protein kinase that in humans is encoded by the CDK12 gene. This enzyme is a member of cyclin-dependent kinase protein family. CDK12 forms protein complexes with cyclin K (CCNK) . The CDK12-CCNK complex acts at the level of RNA polymerase II (RNAPII) to regulate expression of long complex genes, several of which have roles in DNA maintenance and repair.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL, Immunoprecipitation: 2ug antibody/500ug lysate

UniProt

Q9NYV4

Host

Rabbit

Reactivity

Human

Immunogen

A human recombinant partial protein (amino acids Q1147-N1439) was used as the immunogen for the CDK12 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, IHC-P, IP, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the CDK12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CDK12 antibody is available for research use only.

Storage Conditions

After reconstitution, the CDK12 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CDK12 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunofluorescent staining of FFPE human A549 cells with CDK12 antibody (green) and Beta Tubulin mAb (red) . HIER: steam section in pH6 citrate buffer for 20 min.