Keratin 18 Antibody

Keratin 18, mapped to 12q13.13, is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 2-4 µg/mL

UniProt

P05783

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein (amino acids E204-H430) was used as the immunogen for the Keratin 18 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

7I6

Applications

WB, IHC-P, FACS, IF

Purity

Affinity purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Keratin 18 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Keratin 18 antibody is available for research use only.

Storage Conditions

After reconstitution, the Keratin 18 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Keratin 18 antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human placental tissue with Keratin 18 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA for 20 min.