ZAP70 Antibody

ZAP-70 (Zeta-chain-associated protein kinase 70) encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P43403

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Amino acids MRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQL from the human protein were used as the immunogen for the ZAP70 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

6F12

Applications

WB, FACS

Purity

Affinity purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the ZAP70 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ZAP70 antibody is available for research use only.

Storage Conditions

After reconstitution, the ZAP70 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ZAP70 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human 293T cells with ZAP70 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= ZAP70 antibody.