NDRG1 Antibody

Protein NDRG1 is a protein that in humans is encoded by the NDRG1 gene. It is a member of the N-myc downregulated gene family which belong to the alpha/beta hydrolase superfamily. The NDRG1 gene is mapped to chromosome 8q24. It has got a deduced 394 amino acid protein with a molecular mass of 43kD. NDRG1 is expressed in the cytoplasm and basolateral membranes of gut lumen surface epithelial cells. The protein appears to play a vital role in growth arrest and cell differentiation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q92597

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids S2-N220) was used as the immunogen for the NDRG1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the NDRG1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NDRG1 antibody is available for research use only.

Storage Conditions

After reconstitution, the NDRG1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NDRG1 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human PC-3 cells with NDRG1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= NDRG1 antibody.