MC4-R Antibody / MC4R / Melanocortin Receptor 4

Melanocortin receptor 4 is a protein that in humans is encoded by the MC4R gene. This gene is mapped to 18q21.32. The protein encoded by this gene is a membrane-bound receptor and member of the melanocortin receptor family. The encoded protein interacts with adrenocorticotropic and MSH hormones and is mediated by G proteins. This is an intronless gene. Defects in this gene are a cause of autosomal dominant obesity.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P32245

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids QELRKTFKEIICCYPLGGLCDLSSRY from the human protein were used as the immunogen for the MC4-R antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the MC4-R antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MC4-R antibody is available for research use only.

Storage Conditions

After reconstitution, the MC4-R antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MC4-R antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human A431 cells with MC4-R antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.