ATP5H Antibody
ATP5H is also known as ATPQ. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8) . This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL
UniProt
O75947
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Recombinant human protein (amino acids A2-L161) was used as the immunogen for the ATP5H antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
6B12
Applications
WB, IHC-P, IF
Purity
Affinity purified
Format
Purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
Limitations
This ATP5H antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ATP5H antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human breast cancer with ATP5H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items