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ATP5H Antibody

ATP5H is also known as ATPQ. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8) . This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL

UniProt

O75947

Host

Mouse

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant human protein (amino acids A2-L161) was used as the immunogen for the ATP5H antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

6B12

Applications

WB, IHC-P, IF

Purity

Affinity purified

Format

Purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the ATP5H antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This ATP5H antibody is available for research use only.

Storage Conditions

After reconstitution, the ATP5H antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the ATP5H antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human breast cancer with ATP5H antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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