MED4 Antibody

Mediator of RNA polymerase II transcription subunit 4 also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36) is a protein that in humans is encoded by the MED4 gene. It is mapped to 13q14.2. This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9NPJ6

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids N24-D254) was used as the immunogen for the MED4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the MED4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MED4 antibody is available for research use only.

Storage Conditions

After reconstitution, the MED4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MED4 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human rectal cancer with MED4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

MBS1394455-01 0.02 mg (E-Coli)

Recombinant Pseudomonas aeruginosa 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)

Ask

View Details

MBS1394455-02 0.02 mg (Yeast)

Recombinant Pseudomonas aeruginosa 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)

Ask

View Details

MBS1394455-03 0.1 mg (E-Coli)

Recombinant Pseudomonas aeruginosa 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)

Ask

View Details

MBS1394455-04 0.1 mg (Yeast)

Recombinant Pseudomonas aeruginosa 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)

Ask

View Details

MBS1394455-05 0.02 mg (Baculovirus)

Recombinant Pseudomonas aeruginosa 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)

Ask

View Details

MBS1394455-06 0.02 mg (Mammalian-Cell)

Recombinant Pseudomonas aeruginosa 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)

Ask

View Details