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GAP43 Antibody

Growth Associated Protein 43 (GAP43) is a protein encoded by the GAP43 gene in humans. It is mapped to 3q13.31. The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL

UniProt

P17677

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids IKPEDKAHKAATKIQASFRGH from the human protein were used as the immunogen for the GAP43 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IHC-P, IF

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the GAP43 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This GAP43 antibody is available for research use only.

Storage Conditions

After reconstitution, the GAP43 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the GAP43 antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE rat brain with GAP43 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA for 20 min.

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