MMUT Antibody / Methylmalonyl Coenzyme A Mutase
Methylmalonyl-CoA mutase (MUT) is a mitochondrial enzyme that catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA. This gene is mapped to 6p12.3. MUT requires a vitamin B12-derived prosthetic group, adenosylcobalamin (commonly referred to as AdoCbl), to function. And the product of this enzyme, succinyl-CoA, is a key molecule of the TCA cycle.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL
UniProt
P22033
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids LHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPE from the human protein were used as the immunogen for the MMUT antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
2D6
Applications
WB, IHC-P
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
Limitations
This MMUT antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the MMUT antibody should be determined by the researcher.
Location
Cytoplasmic (mitochondria)
Image Legend
IHC staining of FFPE human rectal cancer with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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