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MMUT Antibody / Methylmalonyl Coenzyme A Mutase

Methylmalonyl-CoA mutase (MUT) is a mitochondrial enzyme that catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA. This gene is mapped to 6p12.3. MUT requires a vitamin B12-derived prosthetic group, adenosylcobalamin (commonly referred to as AdoCbl), to function. And the product of this enzyme, succinyl-CoA, is a key molecule of the TCA cycle.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL

UniProt

P22033

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids LHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPE from the human protein were used as the immunogen for the MMUT antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

2D6

Applications

WB, IHC-P

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the MMUT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MMUT antibody is available for research use only.

Storage Conditions

After reconstitution, the MMUT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MMUT antibody should be determined by the researcher.

Location

Cytoplasmic (mitochondria)

Image Legend

IHC staining of FFPE human rectal cancer with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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