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Rad9b Antibody

By coimmunoprecipitation of erythroleukemia cells transfected with RAD9B, it was found that RAD9B associated with 3 endogenous checkpoint proteins, HUS1, RAD1, and RAD17, possibly in a 9-1-1-like complex. RAD9B also interacted with transfected HUS1B. It was also found decreased abundance of RAD9B mRNA in testicular seminomas relative to normal tissue controls and other types of testicular tumors.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q6WBX7

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Recombinant mouse protein (amino acids M1-G403) was used as the immunogen for the Rad9b antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Rad9b antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Rad9b antibody is available for research use only.

Storage Conditions

After reconstitution, the Rad9b antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Rad9b antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE rat brain with Rad9b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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