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TRIM33 Antibody / TIF1 gamma

Tripartite motif-containing 33 (TRIM33), also known as transcriptional intermediary factor 1 gamma (TIF1g), is a human gene. The TRIM33 gene is mapped to chromosome 1p13 by FISH. The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described; however, the full-length nature of one variant has not been determined.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q9UPN9

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein (amino acids M1001-K1127) was used as the immunogen for the TRIM33 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

8I8

Applications

WB, IHC-P, IF, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the TRIM33 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TRIM33 antibody is available for research use only.

Storage Conditions

After reconstitution, the TRIM33 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TRIM33 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human breast cancer with TRIM33 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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