Gilt Antibody / Ifi30

Gamma-interferon-inducible lysosomal thiol reductase is an enzyme that, in humans, is encoded by the IFI30 gene. The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL

UniProt

Q9ESY9

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Amino acids QLMHENAQLTDALHPPHEY from the mouse protein were used as the immunogen for the Gilt antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Gilt antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Gilt antibody is available for research use only.

Storage Conditions

After reconstitution, the Gilt antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Gilt antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE mouse lymph tissue with Gilt antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.