B-ARK1 Antibody / GRK2

Beta adrenergic receptor kinase (beta-ARK or BARK) is a serine/threonine intracellular kinase. The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 2-4 µg/mL

UniProt

P25098

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Amino acids KKELRDAYREAQQLVQRV from the human protein were used as the immunogen for the B-ARK1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IF

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the B-ARK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This B-ARK1 antibody is available for research use only.

Storage Conditions

After reconstitution, the B-ARK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the B-ARK1 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human U937 cells with B-ARK1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= B-ARK1 antibody.