TRPV4 Antibody

TRPV4 (Transient Receptor Potential Cation Channel Subfamily V Member 4), also known as TRP12, OTRPC4 or VROAC, is a protein that in humans is encoded by the TRPV4 gene. This gene encodes TRPV4, a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. By genomic sequence analysis, Liedtke et al. (2000) mapped the TRPV4 gene to chromosome 12q24.1. They mapped the mouse Trpv4 gene to distal chromosome 5 by radiation hybrid analysis. Wissenbach et al. (2000) found that hypoosmotic conditions rapidly activated TRP12, while hyperosmotic conditions inhibited the activity. Strotmann et al. (2000) showed that OTRPC4 was responsive to changes in extracellular osmolarity in the physiologically relevant range and was expressed in tissues exposed to changing osmotic environments.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9HBA0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids N66-L871) was used as the immunogen for the TRPV4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the TRPV4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TRPV4 antibody is available for research use only.

Storage Conditions

After reconstitution, the TRPV4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TRPV4 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) human HEK293, 2) rat PC-3, 3) rat C6, 4) mouse brain and 5) mouse testis lysate with TRPV4 antibody. Expected molecular weight: 90-110 kDa depending on level of glycosylation.