MitoNEET Antibody / CISD1
CISD1, also called MitoNEET, is a member of the CDGSH domain-containing family and may play a role in the regulation of mitochondrial oxidative capacity. This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q9NZ45
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids Y28-E93) was used as the immunogen for the MitoNEET antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
Limitations
This MitoNEET antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the MitoNEET antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human gastric cancer with MitoNEET antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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