CXCR2 Antibody

CXCR2 is a receptor for Interleukin 8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family (subfamily, chemokine) . Binding of IL8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL8 with a high affinity and to GRO/MGSA and NAP2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P25025

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids TLPPFLLDAAPCEPESLEINK from the human protein were used as the immunogen for the CXCR2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, FACS

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the CXCR2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CXCR2 antibody is available for research use only.

Storage Conditions

After reconstitution, the CXCR2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CXCR2 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human PBM cells with CXCR2 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= CXCR2 antibody.