Beta Tubulin Antibody

Tubulin beta chain is a protein that in humans is encoded by the TUBB gene. This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/10^6 cells, Immunocytochemistry: 2-4 µg/mL

UniProt

P07437

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids EQFTAMFRRKAFLHWYTGEGMDEMEFTEAE were used as the immunogen for the Beta Tubulin antibody.

Clonality

Monoclonal

Isotype

IgG2a

Clone

2E11.

Applications

WB, FACS, ICC

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Beta Tubulin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Beta Tubulin antibody is available for research use only.

Storage Conditions

After reconstitution, the Beta Tubulin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Beta Tubulin antibody should be determined by the researcher.

Image Legend

IF/ICC staining of FFPE human U-2 OS cells with Beta Tubulin antibody (green) at 2ug/ml and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.