HIF-1 alpha Antibody

HIF-1a (Hypoxia-inducible factor 1 alpha or HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF-1a transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. HIF-1alpha is over expressed during carcinogenesis, myocardial infarction and wound healing. It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers, resulting in the activation of genes essential for cell survival. HIF-1a regulates the survival and function in the inflammatory microenvironment directly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.25-0.5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q16665

Host

Rabbit

Reactivity

Human

Immunogen

A human recombinant protein (amino acids H197-R718) was used as the immunogen for the HIF-1 alpha antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the HIF-1 alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This HIF-1 alpha antibody is available for research use only.

Storage Conditions

After reconstitution, the HIF-1 alpha antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the HIF-1 alpha antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) U-87 MG, 2) U-2 OS, 3) PC-3, 4) A549 and 5) HepG2 lysate with HIF-1 alpha antibody. Routinely observed molecular weight: 100~120 kDa.