CD90 Antibody

CD90 (Cluster of Differentiation 90) or Thy-1 is a heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. The CD90 gene is mapped to 11q23.3. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P04216

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant protein (amino acids Q20-H111) was used as the immunogen for the CD90 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the CD90 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CD90 antibody is available for research use only.

Storage Conditions

After reconstitution, the CD90 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CD90 antibody should be determined by the researcher.

Image Legend

Western blot testing of 1) rat brain, 2) rat thymus and 3) mouse brain lysate with CD90 antibody. Expected molecular weight 18~35 kDa depending on glycosylation level.