MCM2 Antibody

MCM2 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 2), also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division. And it is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunocytochemistry: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P49736

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant protein (amino acids S393-R850) was used as the immunogen for the MCM2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, ICC, FACS, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the MCM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This MCM2 antibody is available for research use only.

Storage Conditions

After reconstitution, the MCM2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the MCM2 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IF/ICC staining of FFPE human A431 cells with MCM2 antibody (green) at 2ug/ml and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.