Beta Glucuronidase Antibody / Gusb

Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 2-4 µg/mL, Direct ELISA: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 5 µg/mL

UniProt

P08236

Host

Rabbit

Reactivity

Human

Immunogen

A human recombinant protein (amino acids Q416-T651) was used as the immunogen for the Beta Glucuronidase antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF/ICC, FACS, IHC-P, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Beta Glucuronidase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Beta Glucuronidase antibody is available for research use only.

Storage Conditions

After reconstitution, the Beta Glucuronidase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Beta Glucuronidase antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFEP human A431 cells with Beta Glucuronidase antibody (red) and DAPI nuclear stain (blue) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.