Exonuclease 1 Antibody / EXO1

Exonuclease 1 is an enzyme that in humans is encoded by the EXO1 gene. It is mapped to 1q43. This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9UQ84

Host

Rabbit

Reactivity

Human

Immunogen

A human recombinant protein (amino acids M1-K294) was used as the immunogen for the Exonuclease 1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Exonuclease 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Exonuclease 1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Exonuclease 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Exonuclease 1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human spleen tissue with Exonuclease 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.