Thioredoxin 2 Antibody

Thioredoxin, mitochondrial also known as thioredoxin-2 is a protein that in humans is encoded by the TXN2 gene on chromosome 22. It is mapped to 22q12.3. This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q99757

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant protein (amino acids T60-G166) was used as the immunogen for the Thioredoxin 2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF FACS, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the Thioredoxin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Thioredoxin 2 antibody is available for research use only.

Storage Conditions

After reconstitution, the Thioredoxin 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Thioredoxin 2 antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) HeLa, 2) K562, 3) ThP-1, 4) U-2 OS and 5) U-87 MG cell lysate with Thioredoxin 2 antibody. Predicted molecular weight ~13 kDa.