TJP1 Antibody / ZO-1

Tight junction protein ZO-1 is a protein that in humans is encoded by the TJP1 gene. It is mapped to 15q13.1. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. It has been found that injected CagA associates with the epithelial tight-junction scaffolding protein TJP1 and the transmembrane protein junctional adhesion molecule, causing an ectopic assembly of tight junction components at sites of bacterial attachment, and altering the composition and function of the apical-junctional complex.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 2-4 µg/mL

UniProt

Q07157

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant protein (amino acids H1178-F1527) was used as the immunogen for the TJP1 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

3E12.

Applications

WB, IHC-P, FACS, IF

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the TJP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TJP1 antibody is available for research use only.

Storage Conditions

After reconstitution, the TJP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TJP1 antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human kidney cancer with TJP1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.