DCC Antibody

Deleted in Colorectal Carcinoma, also known as DCC, is a protein which in humans is encoded by the DCC gene. It is mapped to 18q21.2. This gene encodes a netrin 1 receptor. The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK, also known as PTK2) to mediate axon attraction. The protein partially localizes to lipid rafts, and induces apoptosis in the absence of ligand. The protein functions as a tumor suppressor, and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P43146

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant protein (amino acids Q851-F1447) was used as the immunogen for the DCC antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, Direct ELISA

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the DCC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This DCC antibody is available for research use only.

Storage Conditions

After reconstitution, the DCC antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the DCC antibody should be determined by the researcher.

Image Legend

Western blot testing of human 1) U-87 MG, 2) U-2 OS, 3) A431 and 4) A549 cell lysate with DCC antibody. Expected molecular weight: 155~190 kDa depending on glycosylation level.