RAB27A Antibody

Ras-related protein Rab-27A is a protein that in humans is encoded by the RAB27A gene. The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence/Immunocytochemistry: 2-4 µg/mL

UniProt

P51159

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A human recombinant protein (amino acids L98-K216) was used as the immunogen for the RAB27A antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

2F5

Applications

WB, IHC-P, FACS, IF/ICC

Purity

Affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Reconstitution

After reconstitution, the RAB27A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This RAB27A antibody is available for research use only.

Storage Conditions

After reconstitution, the RAB27A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the RAB27A antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human prostate cancer with RAB27A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.